Generator

Part:BBa_K325218:Design

Designed by: Anja Hohmann and Ben Reeve   Group: iGEM10_Cambridge   (2010-10-23)


Orange Firefly Luciferase (under pBAD)
L. Cruciata
(E. coli optimised)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 2322
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2864
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 2378


Design Notes

a


Source

a

References